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pepper mild mottle virus pmmov pv0165  (DSMZ)
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DSMZ pepper mild mottle virus pmmov pv0165
Sensitivity and specificity tests to determine ToMMV limit of detection. (A) Sensitivity was tested using 10-fold serial dilutions of total RNA of ToMMV-infected tomato plants spiked into healthy plant RNA. (B) Specificity of the assay was analyzed using targeted and non-targeted viruses and viroids. 1. ToMMV- (Tomato mottle mosaic virus), 2. TMV- (Tobacco mosaic virus), 3. ToMV- (Tomato mosaic virus), 4. ToBRFV- (Tomato brown rugose fruit virus-PV-1236), 5. TSWV- (Tomato spotted wilt orthotospovirus, 6. ToRSV- (Tomato ringspot virus), 7. ToCV- (Tomato chlorosis virus -PC-1242), 8. TICV- (Tomato infectious chlorosis virus -PV-1108), 9. <t>PMMoV-</t> (Pepper mild mottle virus-PV-0165) 10. BPeMV- (Bell pepper mottle virus -PC-0170), 11. WGMMV (Watermelon green mottle mosaic virus), 12. CGMMV- (Cucumber green mottle mosaic virus). 13. CEVd (Citrus exocortis viroid), 14. NTC (No template control), 15. Healthy control, 16. TCDVd (Tomato chlorotic dwarf viroid), 17. TASVd (Tomato apical stunt viroid), 18. MPVd (Mexican papita viroid), 19. CLVd (Columnea latent viroid), 20. TPMVd (Tomato planta macho viroid), 21. PSTVd (Potato spindle tuber viroid) 22. NTC (no template control), 23. Healthy, 24. positive (ToMMV) control, and L- (Electronic Ladder), NTC- (No template control), H- (Healthy). (C) Validation of the developed RT-PCR method for detection of a diverse collection of ToMMV isolates. Lane M: PCR marker (Promega); Lane 1: South Carolina isolate V13-478 (SC13-05); Lane 2: New York isolate 1 V13-481 (NY13-01) Lane 3: New York isolate 2 V13-481 (NY13-02); Lane 4: California isolate 1 V16-07 (CA16-01); Lane 5: California isolate 2 V16-08 (CA16-02); Lane 6: ToMMV Mexican isolate -MX5; Lane 7: Positive Control 1; Lane 8. Positive Control 2, Lane 9: Healthy plant control.
Pepper Mild Mottle Virus Pmmov Pv0165, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
pepper mild mottle virus pmmov pv0165 - by Bioz Stars, 2026-10
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Sensitivity and specificity tests to determine ToMMV limit of detection. (A) Sensitivity was tested using 10-fold serial dilutions of total RNA of ToMMV-infected tomato plants spiked into healthy plant RNA. (B) Specificity of the assay was analyzed using targeted and non-targeted viruses and viroids. 1. ToMMV- (Tomato mottle mosaic virus), 2. TMV- (Tobacco mosaic virus), 3. ToMV- (Tomato mosaic virus), 4. ToBRFV- (Tomato brown rugose fruit virus-PV-1236), 5. TSWV- (Tomato spotted wilt orthotospovirus, 6. ToRSV- (Tomato ringspot virus), 7. ToCV- (Tomato chlorosis virus -PC-1242), 8. TICV- (Tomato infectious chlorosis virus -PV-1108), 9. PMMoV- (Pepper mild mottle virus-PV-0165) 10. BPeMV- (Bell pepper mottle virus -PC-0170), 11. WGMMV (Watermelon green mottle mosaic virus), 12. CGMMV- (Cucumber green mottle mosaic virus). 13. CEVd (Citrus exocortis viroid), 14. NTC (No template control), 15. Healthy control, 16. TCDVd (Tomato chlorotic dwarf viroid), 17. TASVd (Tomato apical stunt viroid), 18. MPVd (Mexican papita viroid), 19. CLVd (Columnea latent viroid), 20. TPMVd (Tomato planta macho viroid), 21. PSTVd (Potato spindle tuber viroid) 22. NTC (no template control), 23. Healthy, 24. positive (ToMMV) control, and L- (Electronic Ladder), NTC- (No template control), H- (Healthy). (C) Validation of the developed RT-PCR method for detection of a diverse collection of ToMMV isolates. Lane M: PCR marker (Promega); Lane 1: South Carolina isolate V13-478 (SC13-05); Lane 2: New York isolate 1 V13-481 (NY13-01) Lane 3: New York isolate 2 V13-481 (NY13-02); Lane 4: California isolate 1 V16-07 (CA16-01); Lane 5: California isolate 2 V16-08 (CA16-02); Lane 6: ToMMV Mexican isolate -MX5; Lane 7: Positive Control 1; Lane 8. Positive Control 2, Lane 9: Healthy plant control.

Journal: Frontiers in Plant Science

Article Title: Validation of a one-step reverse transcription PCR detection method for Tobamovirus maculatessellati , in tomato ( Solanum lycopersicum L.) and pepper ( Capsicum annuum L.)

doi: 10.3389/fpls.2025.1535175

Figure Lengend Snippet: Sensitivity and specificity tests to determine ToMMV limit of detection. (A) Sensitivity was tested using 10-fold serial dilutions of total RNA of ToMMV-infected tomato plants spiked into healthy plant RNA. (B) Specificity of the assay was analyzed using targeted and non-targeted viruses and viroids. 1. ToMMV- (Tomato mottle mosaic virus), 2. TMV- (Tobacco mosaic virus), 3. ToMV- (Tomato mosaic virus), 4. ToBRFV- (Tomato brown rugose fruit virus-PV-1236), 5. TSWV- (Tomato spotted wilt orthotospovirus, 6. ToRSV- (Tomato ringspot virus), 7. ToCV- (Tomato chlorosis virus -PC-1242), 8. TICV- (Tomato infectious chlorosis virus -PV-1108), 9. PMMoV- (Pepper mild mottle virus-PV-0165) 10. BPeMV- (Bell pepper mottle virus -PC-0170), 11. WGMMV (Watermelon green mottle mosaic virus), 12. CGMMV- (Cucumber green mottle mosaic virus). 13. CEVd (Citrus exocortis viroid), 14. NTC (No template control), 15. Healthy control, 16. TCDVd (Tomato chlorotic dwarf viroid), 17. TASVd (Tomato apical stunt viroid), 18. MPVd (Mexican papita viroid), 19. CLVd (Columnea latent viroid), 20. TPMVd (Tomato planta macho viroid), 21. PSTVd (Potato spindle tuber viroid) 22. NTC (no template control), 23. Healthy, 24. positive (ToMMV) control, and L- (Electronic Ladder), NTC- (No template control), H- (Healthy). (C) Validation of the developed RT-PCR method for detection of a diverse collection of ToMMV isolates. Lane M: PCR marker (Promega); Lane 1: South Carolina isolate V13-478 (SC13-05); Lane 2: New York isolate 1 V13-481 (NY13-01) Lane 3: New York isolate 2 V13-481 (NY13-02); Lane 4: California isolate 1 V16-07 (CA16-01); Lane 5: California isolate 2 V16-08 (CA16-02); Lane 6: ToMMV Mexican isolate -MX5; Lane 7: Positive Control 1; Lane 8. Positive Control 2, Lane 9: Healthy plant control.

Article Snippet: The non-target viruses included tomato spotted wilt virus (TSWV) and tomato ringspot virus (ToRSV) (from Dr. Tzanetakis, University of Arkansas), ToMV-PV0135, ToBRFV-PV1236, tomato chlorosis virus (ToCV)-PC1242, tomato infectious chlorosis virus (TICV)-PV1108, pepper mild mottle virus (PMMoV)-PV0165, and bell pepper mottle virus (BPeMV)-PC0170 positive samples purchased from North Rhine-Westphalia, Germany (DSMZ).

Techniques: Infection, Virus, Control, Biomarker Discovery, Reverse Transcription Polymerase Chain Reaction, Marker, Positive Control